Skip to main content

Colorectal Cancer and Altered Gut Microbiota

Is this the reason that people who consume a diet high in fibre from fruit and vegetables and a lower in animal fat and protein (both animal fat and protein diets attract bacteriodes) have a lower incidence of colon cancer?  It would seem that it is every bit as important to provide the food for our microbiota to thrive on as it is to feed ourselves.  We and our microbiota would appear to benefit mutually from the same types of food - it's not just about nutrition, it's about feeding our "helpful friends" as well!


The Differences in Colonic Mucosal Microbiota Between Normal Individual and Colon Cancer Patients by Polymerase Chain Reaction-denaturing Gradient Gel Electrophoresis

Authors: Huipeng, Wang MD; Lifeng, Gong MD; Chuang, Ge MD; Jiaying, Zhao MD; Yuankun, Cai MD

Abstract


Objective: The aim of this study was to analyze the differences in the intestinal composition between normal individuals and colon cancer patients.

Methods: To establish the criteria for screening a normal individual for colon cancer, human colonic biopsies were obtained at routine colonoscopy. For patients with colon cancer, samples were obtained from cancerous regions. For normal individuals, colonic biopsies were taken from 3 sites of large intestine (descending, transverse, and ascending colon). Thereafter, a comparison of the microbiota structure by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was carried out. At last, bacterial species were identified by sequencing special bands from DGGE gels and comparing data with sequence databases.

Result: With PCR-DGGE, we have discovered that the diversity and richness of the bacterial community from colon cancer patient’s colonic mucosa were lower than that of the normal individual’s sample. Then, a special DGGE band was found in the colon cancer patients. After sequencing, we confirmed that it had a high level of similarity with bacteroides.

Conclusions: Colon cancers are closely related with the alteration of intestinal flora such as the reduction of biodiversity and richness of the bacterial community. Furthermore, the increase in proportion of bacteroides may be directly associated with colon cancer.
Journal of Clinical Gastroenterology:
February 2014 - Volume 48 - Issue 2 - p 138-144
doi: 10.1097/MCG.0b013e3182a26719
ALIMENTARY TRACT: Original Articles


Labels:

Comments

Post a Comment

We welcome your comment and contribution to the fecal microbiota transplant discussion

Popular posts from this blog

Clinics and Doctors Who Perform Fecal Microbiota Transplantation

We are compiling a list of doctors and hospitals around the world who have performed fecal microbiota transplants - the list will grow as more doctors start to use this procedure on their ailing patients. We would love your help in compiling this list, so a comment with a name of a doctor/physician/medical professional and any information you can give would be very much appreciated! Please don't try this procedure without qualified medical guidance and advice. It is essential that fecal doners be screened for various diseases including some which are contageous. This is why a list of doctors who are familiar with the procedure is important, so that those of you who feel fecal microbiota transplantation could be beneficial to you can seek out appropriate medical advice. Doctors from around the world are listed below: Australia Professor Thomas Borody , Centre For Digestive Diseases Five Dock, Sydney USA Dr Lawrence J Brandt Montefi...
66 comments
Read more

"Jake's" Widespread Crohn's Disease Gone Thanks To FMT! 20 November 2013

It's been a while since I posted about my son "Jake".  For those who haven't read his story, here is the link   http://www.fecalmicrobiotatransplant.com/2013/07/crohns-disease-fmt.html  . He has been off medication (Imuran) for nine months now and looks and feels well.  Unfortunately his cousin who was his donor became unsuitable to donate in mid August due to picking up a parasite, so Jake has gone since then without any FMT . Today he had a colonoscopy and endoscopy and we were thrilled with the news.  There is no sign of Crohn's Disease anywhere - not in his intestines, not in his terminal ileum and not in his duodenum!!! In fact, Professor Borody said there was no scarring or ulceration or any indication that the disease had ever existed.  It was all perfect, healthy and inflammation-free.  When he was first diagnosed with Crohn's three years ago it was very widespread.  I am sure that anyone ...
17 comments
Read more

Crohn's Disease, Acne and Fecal Microbiota Transplant In Teenager - Remission Without Medication! 27 July 2013

I have hesitated in sharing my son's story with Crohn's Disease and the success we have had with FMT (fecal microbiota transplantation) until now because I didn't want to do so prematurely. My son is 15 years old.  To protect his privacy, I will call him Jake, although that is not his real name :-). Jake was diagnosed with Crohn's Disease a few days before his 13th birthday.  It was widespread ulceration, inflammation and infection throughout his duodenum, terminal ileum, ascending colon, descending colon and rectum.  It was a "gut-wrenching" time for me as a mother to an only child, especially after being told how very serious this disease is, how extensively he had it, that he would have it for the rest of his life, and probably do poorly in the long-term because he was so young at diagnosis.  I was worried sick with the long term consequences of my son taking immune-suppressive drugs (in his case, Imuran) - on the other hand I was terrified of the c...
18 comments
Read more