Sunday, January 12, 2014

Colorectal Cancer and Altered Gut Microbiota

Is this the reason that people who consume a diet high in fibre from fruit and vegetables and a lower in animal fat and protein (both animal fat and protein diets attract bacteriodes) have a lower incidence of colon cancer?  It would seem that it is every bit as important to provide the food for our microbiota to thrive on as it is to feed ourselves.  We and our microbiota would appear to benefit mutually from the same types of food - it's not just about nutrition, it's about feeding our "helpful friends" as well!

The Differences in Colonic Mucosal Microbiota Between Normal Individual and Colon Cancer Patients by Polymerase Chain Reaction-denaturing Gradient Gel Electrophoresis

Authors: Huipeng, Wang MD; Lifeng, Gong MD; Chuang, Ge MD; Jiaying, Zhao MD; Yuankun, Cai MD


Objective: The aim of this study was to analyze the differences in the intestinal composition between normal individuals and colon cancer patients.

Methods: To establish the criteria for screening a normal individual for colon cancer, human colonic biopsies were obtained at routine colonoscopy. For patients with colon cancer, samples were obtained from cancerous regions. For normal individuals, colonic biopsies were taken from 3 sites of large intestine (descending, transverse, and ascending colon). Thereafter, a comparison of the microbiota structure by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was carried out. At last, bacterial species were identified by sequencing special bands from DGGE gels and comparing data with sequence databases.

Result: With PCR-DGGE, we have discovered that the diversity and richness of the bacterial community from colon cancer patient’s colonic mucosa were lower than that of the normal individual’s sample. Then, a special DGGE band was found in the colon cancer patients. After sequencing, we confirmed that it had a high level of similarity with bacteroides.

Conclusions: Colon cancers are closely related with the alteration of intestinal flora such as the reduction of biodiversity and richness of the bacterial community. Furthermore, the increase in proportion of bacteroides may be directly associated with colon cancer.
Journal of Clinical Gastroenterology:
February 2014 - Volume 48 - Issue 2 - p 138-144
doi: 10.1097/MCG.0b013e3182a26719
ALIMENTARY TRACT: Original Articles

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